This review highlights the recent applications of non-linear optical (NLO) microscopy to study obesity-related health risks. A strong emphasis is given to the applications of coherent anti-Stokes Raman scattering (CARS) microscopy where multiple non-linear optical imaging modalities including CARS, sum-frequency generation (SFG), and two-photon fluorescence are employed simultaneously on a single microscope platform. Specific examples on applications of NLO microscopy to study lipid-droplet biology, obesity-cancer relationship, atherosclerosis, and lipid-rich biological structures are discussed.

中文翻译：

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肥胖相关健康风险的非线性光学成像：回顾


这篇综述重点介绍了非线性光学 (NLO) 显微镜在研究肥胖相关健康风险方面的最新应用。重点强调相干反斯托克斯拉曼散射 (CARS) 显微镜的应用，其中在单个显微镜上同时采用多种非线性光学成像模式，包括 CARS、和频生成 (SFG) 和双光子荧光平台。讨论了应用 NLO 显微镜研究脂滴生物学、肥胖与癌症关系、动脉粥样硬化和富含脂质的生物结构的具体例子。