We describe a high throughput patterning process used to create arrays of molecular-scale features for the study of cytoskeletal protein binding interactions. The process uses a shadow-evaporated metal mask to facilitate lift-off of features defined by nanoimprint lithography. This simple and robust approach alleviates difficulties in pattern transfer of ultra-small features and results in arrays of highly ordered sub-10 nm features which are then functionalized with extracellular matrix proteins. Application of these arrays is demonstrated in cell spreading assays.

中文翻译：

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使用纳米压印光刻技术制备用于研究细胞骨架蛋白结合相互作用的纳米级生物阵列。

我们描述了一种高通量模式化过程，用于创建分子尺度特征阵列以研究细胞骨架蛋白结合相互作用。该工艺使用阴影蒸发金属掩模来促进纳米压印光刻定义的特征的剥离。这种简单而强大的方法减轻了超小特征图案转移的困难，并产生了高度有序的亚 10 nm 特征阵列，然后用细胞外基质蛋白对其进行功能化。这些阵列的应用在细胞扩散试验中得到了证明。