In this contribution, we discuss state-of-the-art methodology for the collection and analysis of hyperspectral images of tissue that will become useful in complementing classical histopathology. In particular, we discuss sampling strategies, data collection methods, and computational approaches to produce pseudo-color maps of large tissue sections of lymph nodes, up to about 100 mm(2) in size. The latter efforts include methods to reduce the presence of dispersion artifacts in IR transflection micro-spectra which can greatly impact the statistical analyzes performed on the data, such as hierarchical cluster analysis and principal components analysis.

中文翻译：

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淋巴结的红外光谱成像：分析和减少伪影的策略


在这篇文章中，我们讨论了收集和分析组织高光谱图像的最先进方法，这将有助于补充经典组织病理学。特别是，我们讨论了采样策略、数据收集方法和计算方法，以生成淋巴结大组织切片的伪彩色图，尺寸可达约 100 mm(2)。后者的努力包括减少红外透反射微光谱中色散伪影的存在的方法，这会极大地影响对数据进行的统计分析，例如层次聚类分析和主成分分析。