Immortalized cell lines, such as human cancer cell lines, are an indispensable experimental resource for many types of biological and medical research. However, unless the cell line has been authenticated prior to use, interpretation of experimental results may be problematic. The potential problems this may cause are illustrated by studies in which authentication of cell lines has not been carried out. For example, immortalized cell lines may unknowingly be infected with viruses that alter their characteristics. In fact, parainfluenza virus type 5 (PIV5) poses a threat to the use of immortalized cell lines in biological and medical research; PIV5 infection significantly alters cellular physiology associated with the response to interferon. If PIV5 infection is widespread in immortalized cell lines, then a very large number of published studies might have to be re-evaluated. Fortunately, analyses of a large number of immortalized cell lines indicate that PIV5 infection is not widespread.

中文翻译：

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副流感病毒对人类癌细胞系是一种威胁病毒吗？

永生化细胞系，如人类癌细胞系，是许多类型的生物和医学研究不可或缺的实验资源。然而，除非细胞系在使用前已经过验证，否则对实验结果的解释可能会有问题。这可能导致的潜在问题通过尚未进行细胞系鉴定的研究加以说明。例如，永生化细胞系可能在不知不觉中感染了改变其特征的病毒。事实上，副流感病毒 5 型 (PIV5) 对生物和医学研究中永生化细胞系的使用构成了威胁；PIV5 感染显着改变了与干扰素反应相关的细胞生理学。如果 PIV5 感染在永生化细胞系中普遍存在，那么大量已发表的研究可能需要重新评估。幸运的是，对大量永生化细胞系的分析表明 PIV5 感染并不普遍。