A cell line designated as NEYS was established from ovarian carcinosarcoma (stage IIIc) of a 56-year-old Japanese woman. The extirpated original tumor was carried in growth medium at 0 °C to the culture room. The primary culture was done on 20 August 2003. The cell line was composed of angular adhesive cells and showed neoplastic and pleomorphic features, such as bizarre aggregation of chromatin granules, an irregular thickening nuclear membrane and multiple large nucleoli. They grew as multi-layered cultures without contact inhibition. The cells proliferated moderately, and population doubling time was about 56 h. The chromosome number showed an underdiploidy of aneuploidy. The modal chromosome numbers were 37 (36%) and 38 (26%). The cultures produced carcinoembryonic antigen (27.4 ng/mL), carbohydrate antigen 19-9 (210 U/mL), and carbohydrate antigen 125 (526 U/mL). The NEYS cells did not give rise to transplant tumors in nude mice, and showed no susceptibility against cisplatin (CDDP), CPT-11, carboplatin, Paclitaxel, Taxotere and 5-FU. This cell line is useful for studies on the histogenesis of carcinosarcoma and susceptibility of cancer drugs in human ovarian carcinosarcoma. The immunohistochemical and ultrastructual analysis demonstrated that NEYS cells showed epithelial and mesenchymal differentiation, and supported the metaplasis theory as the cause of carcinosarcoma.

中文翻译：

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人卵巢癌肉瘤NEYS细胞系的建立和表征，特别是结合抗癌药物的药敏试验。

命名为 NEYS 的细胞系是从一名 56 岁日本女性的卵巢癌肉瘤（IIIc 期）中建立的。将切除的原始肿瘤在0°C的生长培养基中带到培养室。原代培养于2003年8月20日完成。细胞系由角状黏附细胞组成，呈现肿瘤性和多形性特征，如染色质颗粒的奇异聚集、不规则增厚的核膜和多个大核仁。它们以多层培养物的形式生长，没有接触抑制。细胞适度增殖，群体倍增时间约为56小时。染色体数目显示为非整倍体的二倍体。模态染色体数分别为 37 (36%) 和 38 (26%)。培养物产生癌胚抗原 (27.4 ng/mL)、碳水化合物抗原 19-9 (210 U/mL)、和碳水化合物抗原 125 (526 U/mL)。NEYS细胞未在裸鼠体内产生移植瘤，对顺铂（CDDP）、CPT-11、卡铂、紫杉醇、泰索帝和5-FU无敏感性。该细胞系可用于研究癌肉瘤的组织发生和人类卵巢癌肉瘤中抗癌药物的敏感性。免疫组织化学和超微结构分析表明，NEYS 细胞表现出上皮和间充质分化，支持化生作为癌肉瘤病因的理论。该细胞系可用于研究癌肉瘤的组织发生和人类卵巢癌肉瘤中抗癌药物的敏感性。免疫组织化学和超微结构分析表明，NEYS 细胞表现出上皮和间充质分化，支持化生作为癌肉瘤病因的理论。该细胞系可用于研究癌肉瘤的组织发生和人类卵巢癌肉瘤中抗癌药物的敏感性。免疫组织化学和超微结构分析表明，NEYS 细胞表现出上皮和间充质分化，支持化生作为癌肉瘤病因的理论。