There is growing evidence that the human amnion contains various types of stem cells. As amniotic tissue is readily available, it has the potential to be an important source of material for regenerative medicine. In the present study, we evaluated the potential of human amnion-derived fibroblast-like (HADFIL) cells to differentiate into pancreatic islet cells. Two HADFIL cell populations, derived from two different neonates, were analyzed. The expression of pancreatic cell-specific genes was examined before and after in vitro induction of cellular differentiation. We found that Pdx-1, Isl-1, Pax-4, and Pax-6 showed significantly increased expression following the induction of differentiation. In addition, immunostaining demonstrated that insulin, glucagon, and somatostatin were present in HADFIL cells following the induction of differentiation. These results indicate that HADFIL cell populations have the potential to differentiate into pancreatic islet cells. Although further studies are necessary to determine whether such in vitro-differentiated cells can function in vivo as pancreatic islet cells, these amniotic cell populations might be of value in therapeutic applications that require human pancreatic islet cells.

中文翻译：

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从人羊膜来源的成纤维细胞样细胞诱导胰腺样细胞的体外分化。

越来越多的证据表明人类羊膜含有各种类型的干细胞。由于羊膜组织很容易获得，它有可能成为再生医学的重要材料来源。在本研究中，我们评估了人羊膜衍生的成纤维细胞样 (HADFIL) 细胞分化为胰岛细胞的潜力。分析了来自两个不同新生儿的两个 HADFIL 细胞群。在体外诱导细胞分化之前和之后检查胰腺细胞特异性基因的表达。我们发现Pdx-1、Isl-1、Pax-4和Pax-6在诱导分化后表现出显着增加的表达。此外，免疫染色表明，在诱导分化后，HADFIL 细胞中存在胰岛素、胰高血糖素和生长抑素。这些结果表明 HADFIL 细胞群具有分化成胰岛细胞的潜力。尽管需要进一步的研究来确定这种体外分化的细胞是否可以在体内作为胰岛细胞发挥作用，但这些羊膜细胞群在需要人胰岛细胞的治疗应用中可能是有价值的。