UNLABELLED The expression of matrix metallo-proteinases (MMP-7 and MMP-9) and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2), which are involved in the degradation of the extracellular matrix (ECM) and tumor growth, was investigated in normal lung tissue, tissue of benign pulmonary diseases and non-small cell lung cancer (NSCLC) tissue. PATIENTS AND METHODS Tumor tissue and surrounding carcinoma-free lung tissue samples were obtained from 91 patients with NSCLC who had undergone surgery in the years 2005-2007 as well as lung tissue from 12 patients operated on for 'benign' bullous emphysema or interstitial lung disease. The mRNA was isolated from the tissues and the expression of mRNA was assessed using a real-time RT PCR method. RESULTS Significantly higher expression of MMP-7, MMP-9 and TIMP-1 mRNA was demonstrated in the NSCLC tissue in comparison with the normal lung tissue from the same patients (p=0.0003, p<0.0001 and p=0.0018, respectively). Similar results for MMP-7, MMP-9 and TIMP-1 were found in the histological subgroups: squamous cell lung cancer vs. normal tissue (p=0.0198, p=0.0015 and p=0.0366, respectively), and adenocarcinoma vs. normal tissue (p=0.0045, p<0.0001 and p=0.0140, respectively). The expression of MMP-7 was found to be significantly higher in tumor tissue vs. lung tissue of the benign diseases (p=0.0086) and similar results were also recorded in the histological subgroups: squamous cell lung cancer vs. benign tissue (p=0.0171) and adenocarcinoma vs. benign tissue (p=0.0135). The expression of MMP-9 was significantly higher only in the adenocarcinoma subgroup vs. the benign tissue (p=0.0412). No differences in the expression of mRNA between stage IA and stages IB-IIIB of NSCLC were recorded. CONCLUSION Significantly higher expression of MMP-7 and MMP-9 in tumor tissue than in the surrounding tissue or in benign lung disease tissue supports the notion of an important role of these metalloproteinases in the growth of lung carcinoma. TIMP-1 expression is increased only in carcinoma, but not in benign lung disease.

中文翻译：

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非小细胞肺癌（NSCLC）合并肺良性疾病患者肺组织中MMP-7，MMP-9，TIMP-1和TIMP-2 mRNA的表达。

基质金属蛋白酶（MMP-7和MMP-9）和金属蛋白酶组织抑制剂（TIMP-1和TIMP-2）的表达与细胞外基质（ECM）的降解和肿瘤的生长有关。在正常肺组织，良性肺部疾病组织和非小细胞肺癌（NSCLC）组织中进行了研究。患者和方法从2005年至2007年接受手术的91例NSCLC患者以及12例因“良性”大疱性肺气肿或间质性肺病而接受手术的患者的肺组织中获取肿瘤组织和周围无癌的肺组织样本。从组织中分离出mRNA，并使用实时RT PCR方法评估mRNA的表达。结果MMP-7的表达明显升高，与来自相同患者的正常肺组织相比，在NSCLC组织中证实了MMP-9和TIMP-1 mRNA的表达（分别为p = 0.0003，p <0.0001和p = 0.0018）。在组织学亚组中发现MMP-7，MMP-9和TIMP-1的结果相似：鳞状细胞肺癌与正常组织（分别为p = 0.0198，p = 0.0015和p = 0.0366），腺癌与正常组织组织（分别为p = 0.0045，p <0.0001和p = 0.0140）。发现MMP-7在肿瘤组织中的表达明显高于良性疾病的肺组织（p = 0.0086），并且在组织学亚组中也记录了类似的结果：鳞状细胞肺癌与良性组织（p = 0.0171）和腺癌与良性组织（p = 0.0135）。仅在腺癌亚组中，MMP-9的表达明显高于良性组织（p = 0。0412）。在NSCLC的IA期和IB-IIIB期之间，没有观察到mRNA表达的差异。结论肿瘤组织中MMP-7和MMP-9的表达明显高于周围组织或良性肺疾病组织，这支持了这些金属蛋白酶在肺癌生长中的重要作用。TIMP-1表达仅在癌组织中增加，而在肺良性疾病中则没有。