This article examines the current status of Markov processes in single molecule fluorescence. For molecular dynamics to be described by a Markov process, the Markov process must include all states involved in the dynamics and the FPT distributions out of those states must be describable by a simple exponential law. The observation of non-exponential first-passage time distributions or other evidence of non-Markovian dynamics is common in single molecule studies and offers an opportunity to expand the Markov model to include new dynamics or states that improve understanding of the system.

中文翻译：

---

COCIS：单分子荧光中的马尔可夫过程。

本文研究了单分子荧光中马尔可夫过程的现状。对于由马尔可夫过程描述的分子动力学，马尔可夫过程必须包括动力学中涉及的所有状态，并且这些状态之外的 FPT 分布必须可以通过简单的指数定律来描述。非指数首过时间分布或非马尔可夫动力学的其他证据的观察在单分子研究中很常见，并提供了扩展马尔可夫模型以包括新的动力学或状态的机会，以提高对系统的理解。