Phages are promising alternatives to antibodies as the biorecognition element in a variety of biosensing applications. In this study, a monolayer of bacteriophage P22 whose tailspike proteins specifically recognize Salmonella serotypes was covalently bound to glass substrates through a bifunctional cross linker 3-aminopropyltrimethoxysilane. The specific binding of Salmonella typhimurium to the phage monolayer was studied by enzyme-linked immunosorbent assay and atomic force microscopy. Escherichia coli and a Gram-positive bacterium Listeria monocytogenes were also studied as control bacteria. The P22 particles show strong binding affinity to Salmonella typhimurium. In addition, the dried P22 monolayer maintained 50% binding capacity to Salmonella typhimurium after a one-week storage time. This is a promising method to prepare phage monolayer coatings on surface plasmon resonance and acoustic biosensor substrates in order to utilize the nascent phage display technology.

中文翻译：

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固定化噬菌体 P22 单层对鼠伤寒沙门氏菌的识别


噬菌体是抗体的有前途的替代品，作为各种生物传感应用中的生物识别元件。在这项研究中，单层噬菌体 P22（其尾刺蛋白特异性识别沙门氏菌血清型）通过双功能交联剂 3-氨丙基三甲氧基硅烷共价结合到玻璃基板上。通过酶联免疫吸附测定和原子力显微镜研究了鼠伤寒沙门氏菌与噬菌体单层的特异性结合。大肠杆菌和革兰氏阳性细菌单核细胞增生李斯特氏菌也作为对照细菌进行了研究。 P22 颗粒对鼠伤寒沙门氏菌表现出很强的结合亲和力。此外，干燥的P22单层在储存一周后仍保持对鼠伤寒沙门氏菌50%的结合能力。这是一种在表面等离子体共振和声学生物传感器基底上制备噬菌体单层涂层的有前途的方法，以利用新兴的噬菌体展示技术。