We report a new deconvolution method for fluorescence lifetime imaging microscopy (FLIM) based on the Laguerre expansion technique. The performance of this method was tested on synthetic and real FLIM images. The following interesting properties of this technique were demonstrated. 1) The fluorescence intensity decay can be estimated simultaneously for all pixels, without a priori assumption of the decay functional form. 2) The computation speed is extremely fast, performing at least two orders of magnitude faster than current algorithms. 3) The estimated maps of Laguerre expansion coefficients provide a new domain for representing FLIM information. 4) The number of images required for the analysis is relatively small, allowing reduction of the acquisition time. These findings indicate that the developed Laguerre expansion technique for FLIM analysis represents a robust and extremely fast deconvolution method that enables practical applications of FLIM in medicine, biology, biochemistry, and chemistry.

中文翻译：

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基于拉盖尔展开技术的荧光寿命成像显微数据分析的超快方法。

我们报告了一种基于拉盖尔展开技术的荧光寿命成像显微镜（FLIM）的新反卷积方法。该方法的性能在合成和真实 FLIM 图像上进行了测试。演示了该技术的以下有趣特性。1) 可以同时估计所有像素的荧光强度衰减，无需先验假设衰减函数形式。2）计算速度极快，比当前算法至少快两个数量级。3）拉盖尔展开系数的估计图为表示FLIM信息提供了一个新的域。4) 分析所需的图像数量相对较少，可以减少采集时间。这些发现表明，开发的用于 FLIM 分析的拉盖尔展开技术代表了一种稳健且极快的反卷积方法，使 FLIM 在医学、生物学、生物化学和化学中的实际应用成为可能。