BAC (bacterial artificial chromosome) clones from the genomic BAC library of the narrow-leafed lupin (Lupinus angustifolius) were used for cytogenetic mapping of mitotic metaphase chromosomes of that species by the BAC-FISH technique. Location of the clones, together with cytogenetic markers localised earlier by FISH (fluorescencein situ hybridisation) and PRINS (primedin situ DNA labelling), was combined with computer-aided chromosome measurements, to construct the first idiogram of the narrow-leafed lupin. The chromosomes are meta- or submetacentric; the mean absolute chromosome lengths range from 1.9 μm to 3.8 μm, and mean relative lengths from 1.6% to 3.3%. Data concerning linkage of resistance to 2 fungal pathogens as well as assignment of the second linkage group to the appropriate chromosome are given for the first time.

中文翻译：

---

使用 FISH、PRINS 和计算机测量染色体对窄叶羽扇豆 (Lupinus angustifolius L.) 进行核型分析。

来自窄叶羽扇豆 ( Lupinus angustifolius )基因组 BAC 文库的 BAC（细菌人工染色体）克隆用于通过 BAC-FISH 技术对该物种的有丝分裂中期染色体进行细胞遗传学定位。克隆的位置，以及早期通过 FISH（荧光原位杂交）和 PRINS（原位引发）定位的细胞遗传标记DNA 标记），结合计算机辅助的染色体测量，构建了窄叶羽扇豆的第一个 idiogram。染色体是中性或亚中性的；平均绝对染色体长度范围从 1.9 μm 到 3.8 μm，平均相对长度从 1.6% 到 3.3%。首次给出了关于对 2 种真菌病原体的抗性连锁以及将第二连锁群分配给适当染色体的数据。