In this review, we summarize original methods for the extraction of quantitative information from confocal images of gene-expression patterns. These methods include image segmentation, the extraction of quantitative numerical data on gene expression, and the removal of background signal and spatial registration. Finally, it is possible to construct a spatiotemporal atlas of gene expression from individual images recorded at each developmental stage. Initially all methods were developed to extract quantitative numerical information from confocal images of segmentation gene expression in Drosophila melanogaster. The application of these methods to Drosophila images makes it possible to reveal new mechanisms in the formation of segmentation gene expression domains, as well as to construct a quantitative atlas of segmentation gene expression. Most image processing procedures can be easily adapted to process a wide range of biological images.

中文翻译：

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从基因表达原位共聚焦图像获取定量数据的方法。


在这篇综述中，我们总结了从基因表达模式的共焦图像中提取定量信息的原始方法。这些方法包括图像分割、提取基因表达的定量数值数据、去除背景信号和空间配准。最后，可以根据每个发育阶段记录的单个图像构建基因表达的时空图谱。最初，所有方法都是为了从果蝇分割基因表达的共焦图像中提取定量数值信息而开发的。这些方法在果蝇图像中的应用使得揭示分割基因表达域形成的新机制以及构建分割基因表达的定量图谱成为可能。大多数图像处理程序可以轻松地适应处理各种生物图像。