We previously demonstrated that conidia from Aspergillus fumigatus incubated with menadione and paraquat increases activity and expression of cyanide-insensitive alternative oxidase (AOX). Here, we employed the RNA silencing technique in A. fumigatus using the vector pALB1/aoxAf in order to down-regulate the aox gene. Positive transformants for aox gene silencing of A. fumigatus were more susceptible both to an imposed in vitro oxidative stress condition and to macrophages killing, suggesting that AOX is required for the A. fumigatus pathogenicity, mainly for the survival of the fungus conidia during host infection and resistance to reactive oxygen species generated by macrophages.

中文翻译：

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烟曲霉线粒体替代氧化酶基因的沉默增强了活性氧的产生和巨噬细胞对真菌的杀灭。

我们之前已经证明，来自烟曲霉的分生孢子与甲萘醌和百草枯一起孵育会增加对氰化物不敏感的替代氧化酶 (AOX) 的活性和表达。在这里，我们使用载体 pALB1/aoxAf 在烟曲霉中采用 RNA 沉默技术来下调 aox 基因。烟曲霉 aox 基因沉默的阳性转化体对施加的体外氧化应激条件和巨噬细胞杀伤更敏感，表明 AOX 是烟曲霉致病性所必需的，主要是为了在宿主感染期间真菌分生孢子的存活以及对巨噬细胞产生的活性氧的抵抗力。