Platelet storage and availability for the purposes of transfusion are currently restricted by a markedly short shelf life of 5 to 7 days owing to an increased risk of bacterial growth and storage-related deterioration called the platelet storage lesion. Because most bacteria grow to confluence within 5 days during storage at room temperature, there is little increased risk of bacterial overgrowth with testing in place, and the only remaining issue is the quality of platelets during the extended storage. Although the manifestations of the storage lesion have been well studied using a variety of in vitro measures, the precise biochemical pathways involved in the initiation and progression of this process have yet to be identified. Proteomics has emerged as a powerful tool to identify and monitor changes during platelet storage and, in combination with biochemical and physiologic studies, facilitates the development of a sophisticated mechanistic view. In this review, we summarize recent experimental work that has led to a detailed overview of protein changes linked to platelet functions and signaling pathways, providing potential targets for inhibitors to ameliorate the storage lesion.

中文翻译：

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血小板存储病变：从蛋白质组学角度的新认识。

由于细菌生长和与存储相关的退化（称为血小板存储病变）的风险增加，目前用于输血的血小板存储和可用性受到5至7天明显较短的保存期限的限制。由于大多数细菌在室温下存储期间会在5天内生长到汇合，因此在进行适当测试的情况下细菌过度生长的风险几乎没有增加，唯一剩下的问题是长时间存储期间血小板的质量。尽管已使用多种体外方法对储存病变的表现进行了很好的研究，但尚未确定与该过程的发生和发展有关的确切生化途径。蛋白质组学已成为一种强大的工具，可用于识别和监控血小板存储过程中的变化，并且 与生化和生理学研究相结合，有助于发展复杂的机械学观点。在这篇综述中，我们总结了最近的实验工作，这些工作导致了与血小板功能和信号通路相关的蛋白质变化的详细概述，为抑制剂改善存储病变提供了潜在的靶点。