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Effect of quercetin on nicotine-induced biochemical changes and DNA damage in rat peripheral blood lymphocytes.
Redox Report ( IF 5.2 ) Pub Date : 2008-09-18 , DOI: 10.1179/135100008x308948
Shanmugavelu Muthukumaran 1 , Adluri Ram Sudheer , Namasivayam Nalini , Venugopal Padmanabhan Menon
Affiliation  

We elucidated the protective effect of quercetin, a polyphenolic flavonoid, on lipid peroxidation, endogenous antioxidant status and DNA damage during nicotine-induced toxicity in cultured rat peripheral blood lymphocytes as compared to N-acetylcysteine (NAC), a well-known antioxidant. Lymphocytes were exposed to nicotine (3 mM) with and without quercetin and NAC (1 mM) in RPMI-1640 medium for 1 h. In preliminary experiments to fix the effective dose of quercetin, different doses of quercetin (25, 50, 75, 100 and 200 microM) were administered to lymphocytes with nicotine, and lipid peroxidation markers (thiobarbituric acid reactive substances and hydroperoxides) were analysed. A 75 microM dose of quercetin was found to be effective as evidenced by decreased lipid peroxidation. To evaluate the protective potential of quercetin against genotoxic effects of nicotine we used comet and micronucleus assays, which are valid parameters to assess genetic damage. In addition, biochemical changes including lipid peroxidation and antioxidant status were assessed. There were significant increases in the levels of lipid peroxidation, comet parameters and micronuclei frequencies, followed by decrease in the endogenous antioxidant status, in nicotine-treated lymphocytes, which were brought back to near normal by quercetin or NAC treatment. The protective effect of quercetin against nicotine toxicity was comparable to that of NAC. These findings suggest that quercetin can be as effective as NAC in protecting rat peripheral lymphocytes against nicotine-induced cellular and DNA damage.

中文翻译:

槲皮素对尼古丁诱导的大鼠外周血淋巴细胞生化变化和DNA损伤的影响。

我们阐明了槲皮素,一种多酚类黄酮,与众所周知的抗氧化剂N-乙酰半胱氨酸(NAC)相比,对烟碱诱导的培养的大鼠外周血淋巴细胞毒性中脂质过氧化,内源性抗氧化剂状态和DNA损伤的保护作用。在RPMI-1640培养基中,将淋巴细胞暴露于有或没有槲皮素和NAC(1 mM)的尼古丁(3 mM)中。在确定槲皮素有效剂量的初步实验中,对含尼古丁的淋巴细胞施用了不同剂量的槲皮素(25、50、75、100和200 microM),并分析了脂质过氧化标记(硫代巴比妥酸反应性物质和氢过氧化物)。发现脂质减少过氧化可以证明75 microM剂量的槲皮素是有效的。为了评估槲皮素对尼古丁的遗传毒性作用的保护潜力,我们使用了彗星和微核试验,这是评估遗传损伤的有效参数。此外,还评估了包括脂质过氧化和抗氧化剂状态在内的生化变化。尼古丁处理的淋巴细胞中脂质过氧化水平,彗星参数和微核频率显着增加,然后内源性抗氧化剂状态降低,而槲皮素或NAC处理可使淋巴细胞恢复至接近正常水平。槲皮素对尼古丁毒性的保护作用与NAC相当。这些发现表明槲皮素在保护大鼠外周血淋巴细胞免受烟碱诱导的细胞和DNA损伤方面与NAC一样有效。这是评估遗传损伤的有效参数。此外,还评估了包括脂质过氧化和抗氧化剂状态在内的生化变化。尼古丁处理的淋巴细胞中脂质过氧化水平,彗星参数和微核频率显着增加,然后内源性抗氧化剂状态降低,而槲皮素或NAC处理可使淋巴细胞恢复至接近正常水平。槲皮素对尼古丁毒性的保护作用与NAC相当。这些发现表明槲皮素在保护大鼠外周血淋巴细胞免受烟碱诱导的细胞和DNA损伤方面与NAC一样有效。这是评估遗传损伤的有效参数。此外,还评估了包括脂质过氧化和抗氧化剂状态在内的生化变化。尼古丁处理的淋巴细胞中脂质过氧化水平,彗星参数和微核频率显着增加,然后内源性抗氧化剂状态降低,而槲皮素或NAC处理可使淋巴细胞恢复至接近正常水平。槲皮素对尼古丁毒性的保护作用与NAC相当。这些发现表明槲皮素在保护大鼠外周血淋巴细胞免受烟碱诱导的细胞和DNA损伤方面与NAC一样有效。尼古丁处理过的淋巴细胞的脂质过氧化水平,彗星参数和微核频率显着增加,然后内源性抗氧化剂状态降低,通过槲皮素或NAC处理使淋巴细胞恢复至接近正常水平。槲皮素对尼古丁毒性的保护作用与NAC相当。这些发现表明槲皮素在保护大鼠外周血淋巴细胞免受烟碱诱导的细胞和DNA损伤方面与NAC一样有效。尼古丁处理的淋巴细胞中脂质过氧化水平,彗星参数和微核频率显着增加,然后内源性抗氧化剂状态降低,而槲皮素或NAC处理可使淋巴细胞恢复至接近正常水平。槲皮素对尼古丁毒性的保护作用与NAC相当。这些发现表明槲皮素在保护大鼠外周血淋巴细胞免受烟碱诱导的细胞和DNA损伤方面与NAC一样有效。槲皮素对尼古丁毒性的保护作用与NAC相当。这些发现表明槲皮素在保护大鼠外周血淋巴细胞免受烟碱诱导的细胞和DNA损伤方面与NAC一样有效。槲皮素对尼古丁毒性的保护作用与NAC相当。这些发现表明槲皮素在保护大鼠外周血淋巴细胞免受烟碱诱导的细胞和DNA损伤方面与NAC一样有效。
更新日期:2019-11-01
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