The objective of this study was to investigate serum paraoxonase and arylesterase activities, and lipid hydroperoxide (LOOH) and total thiol (total free sulfhydryl groups, -SH) levels along with lipid parameters in patients with knee osteoarthritis. Thirty-six patients with knee osteoarthritis and 30 healthy individuals were enrolled in the study. Serum paraoxonase and arylesterase activities were measured spectrophotometrically. LOOH levels were measured by ferrous oxidation with xylenol orange assay (FOX-2). Serum high-density lipoprotein-cholesterol (HDL-C), -SH levels, paraoxonase and arylesterase activities were significantly lower in the patient group than those in the controls (P < 0.05, for all), while LOOH and low-density lipoprotein (LDL) levels were significantly higher. In conclusion, paraoxonase and arylesterase activities were decreased significantly in patients with knee osteoarthritis. Lower serum paraoxonase-1 activity and lower level of HDL-C seem to be related to increased oxidative stress and inflammatory condition in these patients. It is known that paraoxonases reduce oxidative stress in serum and tissues thereby protecting against cardiovascular disease, particularly atherosclerosis. Thus, decreased paraoxonase and arylesterase activities play a role in the pathogenesis of atherosclerosis through increased susceptibility to lipid peroxidation in patients with osteoarthritis.

中文翻译：

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膝关节骨关节炎患者对氧磷酶活性的评估。

这项研究的目的是调查膝关节骨关节炎患者的血清对氧磷酶和芳基酯酶活性，脂质氢过氧化物（LOOH）和总硫醇（总游离巯基，-SH）水平以及脂质参数。该研究纳入了36例膝关节骨关节炎患者和30名健康个体。分光光度法测定血清对氧磷酶和芳基酯酶的活性。通过二甲酚橙测定法（FOX-2）通过亚铁氧化来测量LOOH水平。患者组的血清高密度脂蛋白胆固醇（HDL-C），-SH水平，对氧磷酶和芳基酯酶活性显着低于对照组（P均<0.05），而LOOH和低密度脂蛋白（P <0.05）。 LDL）水平明显更高。结论，膝骨关节炎患者的对氧磷酶和芳基酯酶活性明显降低。这些患者的血清对氧磷酶-1活性降低和HDL-C水平降低似乎与氧化应激和炎症状况的增加有关。已知对氧磷酶降低血清和组织中的氧化应激，从而预防心血管疾病，特别是动脉粥样硬化。因此，降低的对氧磷酶和芳基酯酶活性通过增加骨关节炎患者对脂质过氧化的敏感性而在动脉粥样硬化的发病机理中起作用。已知对氧磷酶降低了血清和组织中的氧化应激，从而预防了心血管疾病，特别是动脉粥样硬化。因此，降低的对氧磷酶和芳基酯酶活性通过增加骨关节炎患者对脂质过氧化的敏感性而在动脉粥样硬化的发病机理中起作用。已知对氧磷酶降低血清和组织中的氧化应激，从而预防心血管疾病，特别是动脉粥样硬化。因此，降低的对氧磷酶和芳基酯酶活性通过增加骨关节炎患者对脂质过氧化的敏感性而在动脉粥样硬化的发病机理中起作用。