BACKGROUND The rhesus monkey (Macaca mulatta) is a valuable and widely used model animal for biomedical research. However, quantitative analyses of rhesus gene expression profiles under diverse experimental conditions are limited by a shortage of suitable internal controls for the normalization of mRNA levels. In this study, we used a systematic approach for the selection of potential reference genes in the rhesus monkey and compared their suitability to that of the corresponding genes in humans. RESULTS Eight housekeeping genes (HKGs) (GAPDH, SDHA, ACTB, RPL13A, RPL32, UBA52, PGK1Y, and YWHAZ) from rhesus monkeys and humans were selected to test for normalization of expression levels in six different tissue types (brain, colon, kidney, liver, lung, and stomach). Their stability and suitability as reference genes were validated by geNorm, NormFinder and BestKeeper programs. Intriguingly, RPL13A and RPL32 were selected as ideal reference genes only in rhesus monkeys. CONCLUSION The results clearly indicated the necessity of using different reference genes for normalization of expression levels between rhesus monkeys and humans in various tissues.

中文翻译：

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为恒河猴（Macaca mulatta）组织的 SYBR green qRT-PCR 研究选择内参基因。

背景技术恒河猴（Macaca mulatta）是一种有价值且广泛用于生物医学研究的模型动物。然而，在不同实验条件下对恒河猴基因表达谱的定量分析受到缺乏用于标准化 mRNA 水平的合适内部对照的限制。在这项研究中，我们使用系统方法选择恒河猴的潜在参考基因，并将它们的适用性与人类相应基因的适用性进行比较。结果 选择来自恒河猴和人类的 8 个管家基因 (HKG)（GAPDH、SDHA、ACTB、RPL13A、RPL32、UBA52、PGK1Y 和 YWHAZ）来测试六种不同组织类型（脑、结肠、肾）中表达水平的正常化、肝、肺和胃）。它们作为参考基因的稳定性和适用性已通过 geNorm 验证，NormFinder 和 BestKeeper 程序。有趣的是，RPL13A 和 RPL32 仅在恒河猴中被选为理想的参考基因。结论 结果清楚地表明使用不同的参考基因来标准化恒河猴和人类在不同组织中的表达水平的必要性。