Soybean allergy represents a health threat to human and animals. Glycinin and β-conglycinin, the main storage proteins in soybean, have been identified as major food/feed allergens. The present study was conducted to investigate the disappearance of immunoreactive glycinin and β-conglycinin in the digestive processes of piglets. Twelve crossbred piglets, weaned at 21 days of age, were allocated to three dietary treatments in a complete block design, each treatment with four replicates (female/male = 1:1). From day 22–28, the control group was fed diets without leguminous products, while the two treatment groups received diets containing 2.2% purified glycinin or β-conglycinin. All piglets were slaughtered at 29 days of age and digesta was sampled from stomach, middle jejunum, caecum and colon. Results indicated that immunoreactive glycinin and β-conglycinin decreased as the digesta descended down the digestive tract to 0.12% and 0.47%, respectively. Little immunoreactive glycinin was found in the digesta of caecum and colon, while immunoreactive β-conglycinin was detected in the colon. Along the whole digestive tract the disappearance of immunoreactive glycinin was significantly higher than β-conglycinin (p < 0.05).

中文翻译：

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仔猪消化道中免疫反应性大豆球蛋白和𝛃-伴大豆球蛋白的消失

大豆过敏对人类和动物的健康构成威胁。大豆中的主要贮藏蛋白 Glycinin 和 β-conglycinin 已被确定为主要的食物/饲料过敏原。本研究旨在调查免疫反应性大豆球蛋白和 β-伴大豆球蛋白在仔猪消化过程中的消失。12 头 21 日龄断奶的杂交仔猪被分配到完整的区组设计中的三个日粮处理中，每个处理有四个重复（雌性/雄性 = 1:1）。从第 22 天到第 28 天，对照组饲喂不含豆类产品的日粮，而两个治疗组饲喂含有 2.2% 纯化大豆球蛋白或 β-伴大豆球蛋白的日粮。所有仔猪均在 29 日龄时屠宰，并从胃、中空肠、盲肠和结肠中采集食糜样品。结果表明，免疫反应性大豆球蛋白和 β-伴大豆球蛋白随着食糜沿着消化道下降，分别下降至 0.12% 和 0.47%。在盲肠和结肠的食糜中几乎没有发现免疫反应性大豆球蛋白，而在结肠中检测到免疫反应性 β-伴大豆球蛋白。沿着整个消化道，免疫反应性大豆球蛋白的消失显着高于β-伴大豆球蛋白（p < 0.05）。