Glycinin, a major storage protein in soybean, has been identified as an important food allergen. The present study was conducted to investigate the effects of soybean glycinin on the local and systemic immune responses using a swine model specific for glycinin allergy. Performance, peripheral blood lymphocyte proliferation and CD4+ and CD8+ subsets in the plasma of naive and sensitised piglets were determined. In addition, the concentrations of IgA, IgG, IgM, interleukin-4 and interleukin-6 in the jejunum mucosa were measured. Our results showed that dietary supplementation of glycinin reduced piglet performance (p < 0.05), while increasing lymphocyte proliferation and CD4+/CD8+ ratio (p < 0.01). Intestinal mucosal immune responses to glycinin were enhanced with high levels of IgA (p < 0.01) as well as interleukin-4 and interleukin-6 in the jejunum mucosa in glycinin-treated piglets compared with the control (p < 0.01). There were no differences due to treatment in the densities of IgG and IgM. In conclusion, it was found that glycinin stimulated local and systemic immune responses in allergic piglets and had negative effects on piglet performance. The severity of the immune reactions depends on the dose of glycinin with higher doses causing more severe symptoms.

中文翻译：

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大豆甘氨酸对早期断奶仔猪生产性能和免疫功能的影响


大豆球蛋白是大豆中的主要储存蛋白，已被确定为重要的食物过敏原。本研究旨在使用针对大豆球蛋白过敏的猪模型来研究大豆球蛋白对局部和全身免疫反应的影响。测定了幼猪和致敏仔猪的生产性能、外周血淋巴细胞增殖以及血浆中的 CD4+ 和 CD8+ 亚群。另外，测定空肠粘膜中的IgA、IgG、IgM、白细胞介素4、白细胞介素6的浓度。我们的结果表明，日粮中添加大豆球蛋白会降低仔猪生产性能 (p < 0.05)，同时增加淋巴细胞增殖和 CD4+/CD8+ 比率 (p < 0.01)。与对照组相比，经甘氨酸处理的仔猪空肠粘膜中 IgA (p < 0.01) 以及白细胞介素 4 和白细胞介素 6 水平较高 (p < 0.01)，肠粘膜对甘氨酸的免疫反应增强。由于治疗的原因，IgG 和 IgM 的密度没有差异。总之，发现大豆球蛋白刺激过敏仔猪的局部和全身免疫反应，并对仔猪生产性能产生负面影响。免疫反应的严重程度取决于甘氨酸的剂量，剂量越高，症状越严重。