OBJECTIVES Loading of biological matrices offers an opportunity to induce specific cell behaviour. We previously reported the use of growth factors to promote cell invasion and proliferation in tissue valve engineering. We investigated biological matrices preloaded with heparin as an ionically attractive template for the binding, activation and sustained release of basic fibroblast growth factor (bFGF). METHODS Heparin loading concentrations were evaluated and different incubation times were tested. Heparin and heparin-bound bFGF uptake and release were evaluated by (123)I radio-labelling. Biological activity of bFGF was evaluated in vitro. RESULTS Maximum heparin uptake was observed for 2000 microg/ml at 2 h and stabilized thereafter. bFGF-loaded matrices showed an initial burst release of 15% within 4 h and thereafter sustained release reaching 21% at 24 h. Released bFGF was bioactive. CONCLUSIONS This model would be useful in tissue engineering using porcine aortic matrices and could be applied using other growth factors or combinations.

中文翻译：

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在心脏瓣膜组织工程中从负载的支架中持续释放和激活生长因子碱性成纤维细胞生长因子。

目的加载生物基质提供了诱导特定细胞行为的机会。我们先前曾报道在组织瓣膜工程中使用生长因子促进细胞侵袭和增殖。我们调查了预载有肝素作为离子吸引模板的生物基质，以结合，活化和持续释放碱性成纤维细胞生长因子（bFGF）。方法评估肝素负载浓度，并测试不同的孵育时间。肝素和肝素结合的bFGF摄取和释放通过（123）I放射性标记进行评估。在体外评估了bFGF的生物学活性。结果在2 h观察到最大肝素摄取为2000 microg / ml，此后稳定。加载bFGF的基质在4小时内显示了15％的初始爆发释放，此后在24 h持续释放达到21％。释放的bFGF具有生物活性。结论该模型在使用猪主动脉基质的组织工程中将是有用的，并且可以与其他生长因子或组合一起应用。