We compared anabolic and anti-catabolic activities of selected bone morphogenetic proteins (BMP-2, -4, -6, and -7) and cartilage-derived morphogenetic proteins (CDMP-1 and -2) in human normal adult articular chondrocytes. Ankle chondrocytes were cultured in alginate beads in the presence of 10% serum and treated with either growth factors only (each at 100 ng/ml) or the combination of interleukin-1 (IL-1 beta) (0.1 ng/ml) and BMPs. Chondrocyte metabolism was assessed by proteoglycan (PG) synthesis, content, DNA content, and cell survival. The results showed that BMP-2, -4, and -7 were more potent in stimulating PGs than other growth factors tested. The highest levels of PG synthesis were detected at day 9 in the presence of BMP-7. With regard to anti-catabolic properties, the effect depended upon treatment scheme (simultaneous or sequential). Under simultaneous cultures, BMP-2, -4, and -6 failed to counteract IL-1 beta induced inhibition of PG synthesis, while the CDMPs restored this parameter to serum control levels. Only BMP-7 showed consistent and pronounced anti-catabolic activity in either culture treatment scheme. None of the factors induced cell death or chondrocyte proliferation. In conclusion, the growth factors tested showed different levels of effects on human chondrocytes in culture, but only BMP-7 displayed both strong anabolic and anti-catabolic properties.

中文翻译：

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BMPS诱导的人关节软骨细胞培养物中的作用：比较研究。

我们比较了人类正常成人关节软骨细胞中所选骨形态发生蛋白（BMP-2，-4，-6和-7）和软骨衍生形态发生蛋白（CDMP-1和-2）的合成代谢和抗分解代谢活性。在10％血清存在的情况下，在藻酸盐珠中培养踝关节软骨细胞，并仅用生长因子（每种以100 ng / ml的浓度）或白介素-1（IL-1 beta）（0.1 ng / ml）和BMP的组合处理。通过蛋白聚糖（PG）的合成，含量，DNA含量和细胞存活率评估软骨细胞的代谢。结果表明，BMP-2，-4和-7在刺激PG方面比其他测试的生长因子更有效。在BMP-7存在的第9天检测到最高水平的PG合成。关于抗分解代谢性质，效果取决于治疗方案（同时或顺序）。在同时培养的情况下，BMP-2，-4和-6无法抵消IL-1β诱导的PG合成的抑制，而CDMPs将该参数恢复至血清控制水平。在任一培养处理方案中，只有BMP-7表现出一致且明显的抗分解代谢活性。这些因素均未引起细胞死亡或软骨细胞增殖。总之，测试的生长因子对培养的人软骨细胞显示出不同程度的作用，但只有BMP-7表现出强大的合成代谢和抗分解代谢特性。这些因素均未引起细胞死亡或软骨细胞增殖。总之，测试的生长因子对培养的人软骨细胞显示出不同程度的作用，但只有BMP-7表现出强大的合成代谢和抗分解代谢特性。这些因素均未引起细胞死亡或软骨细胞增殖。总之，测试的生长因子对培养的人软骨细胞显示出不同程度的作用，但只有BMP-7表现出强大的合成代谢和抗分解代谢特性。