Unlike mammals, fish have the capacity for functional adult CNS regeneration, which is due, in part, to their ability to express axon growth-related genes in response to nerve injury. One such axon growth-associated gene is gap43, which is expressed during periods of developmental and regenerative axon growth, but is not expressed in CNS neurons that do not regenerate in adult mammals. We previously demonstrated that cis-regulatory elements of gap43 that are sufficient for developmental expression are not sufficient for regenerative expression in the zebrafish. Here we have identified a 3.6kb genomic sequence from Fugu rubripes that can promote reporter gene expression in the nervous system during both development and regeneration in zebrafish. This compact sequence is advantageous for functional dissection of regions important for axon growth-associated gene expression during development and/or regeneration. In addition, this sequence will also be useful for targeting gene expression to neurons during periods of growth and plasticity.

中文翻译：

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来自 Takifugu 的 3.6 kb 基因组序列，能够在斑马鱼神经元发育和再生过程中促进轴突生长相关基因的表达。

与哺乳动物不同，鱼类具有功能性成年中枢神经系统再生的能力，部分原因是它们能够表达轴突生长相关基因以应对神经损伤。一种这样的轴突生长相关基因是 gap43，它在发育和再生轴突生长期间表达，但不在成年哺乳动物中不再生的 CNS 神经元中表达。我们之前已经证明，gap43 的 cis 调节元件足以用于发育表达，但不足以在斑马鱼中进行再生表达。在这里，我们已经确定了一个 3.6kb 的河豚基因组序列，可以在斑马鱼的发育和再生过程中促进神经系统中报告基因的表达。这种紧凑的序列有利于在发育和/或再生过程中对轴突生长相关基因表达重要的区域进行功能解剖。此外，该序列还可用于在生长和可塑性期间将基因表达靶向神经元。