The field of endocytosis is in strong need of formal biophysical modeling and mathematical analysis. At the same time, endocytosis must be much better integrated into cellular physiology to understand the former's complex behavior in such a wide range of phenotypic variations. Furthermore, the concept that endocytosis provides the space-time for signal transduction can now be experimentally addressed. In this review, we discuss these principles and argue for a systematic and top-down approach to study the endocytic membrane system. We provide a summary of published observations on protein kinases regulating endocytic machinery components and discuss global unbiased approaches to further map out kinase regulatory networks. In particular, protein phosphorylation is at the heart of controlling the physical properties of endocytosis and of integrating these physical properties into the signal transduction networks of the cell to allow a fine-tuned response to the continuously varying physiological conditions of a cell.

中文翻译：

---

蛋白激酶：从分子系统的角度出发来研究内吞作用。

内吞作用领域非常需要正式的生物物理建模和数学分析。同时，胞吞作用必须更好地整合到细胞生理学中，以了解前者在如此广泛的表型变异中的复杂行为。此外，现在可以通过实验解决内吞作用为信号转导提供时空的概念。在这篇综述中，我们讨论了这些原理，并提出了一种系统的，自上而下的方法来研究内吞膜系统。我们提供了有关调节内吞机器成分的蛋白激酶的已发表观察结果的摘要，并讨论了进一步阐明激酶调节网络的全球无偏方法。特别是，