Recent scientific discoveries that resulted from the application of next-generation DNA sequencing technologies highlight the striking impact of these massively parallel platforms on genetics. These new methods have expanded previously focused readouts from a variety of DNA preparation protocols to a genome-wide scale and have fine-tuned their resolution to single base precision. The sequencing of RNA also has transitioned and now includes full-length cDNA analyses, serial analysis of gene expression (SAGE)-based methods, and noncoding RNA discovery. Next-generation sequencing has also enabled novel applications such as the sequencing of ancient DNA samples, and has substantially widened the scope of metagenomic analysis of environmentally derived samples. Taken together, an astounding potential exists for these technologies to bring enormous change in genetic and biological research and to enhance our fundamental biological knowledge.

中文翻译：

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下一代DNA测序方法。

下一代DNA测序技术的应用产生的最新科学发现凸显了这些大规模并行平台对遗传学的惊人影响。这些新方法将先前集中的读数从各种DNA制备方案扩展到了全基因组范围，并将其分辨率微调至单碱基精度。RNA的测序也已经过渡，现在包括全长cDNA分析，基于基因表达（SAGE）的系列分析方法以及非编码RNA发现。下一代测序还使诸如古代DNA样品测序之类的新应用成为可能，并且大大拓宽了对环境衍生样品的宏基因组学分析的范围。在一起