PRIMARY OBJECTIVE Granulocyte-colony stimulating factor (G-CSF) is used for the mobilization of bone marrow and endothelial progenitor cells, though G-CSF-induced inflammation may cause endothelial dysfunction. We examined the effects of G-CSF on endothelium, C-reactive protein (CRP), tumour necrosis factor-alpha (TNF-alpha) and anti-inflammatory cytokines namely interleukin 10 (IL-10). RESEARCH DESIGN We studied 60 women with breast cancer, who were randomized to either subcutaneous G-CSF (5 microg/kg), o.d. for 5 days after adjuvant chemotherapy (n = 40) or placebo (n = 20). EXPERIMENTAL INTERVENTIONS We measured flow-mediated dilatation (FMD%) of the brachial artery by ultrasonography, CRP, TNF-alpha, IL-10 and the ratio TNF-alpha/ IL-10 blood levels before, 2-h and 5-days after the G-CSF or placebo treatment. MAIN OUTCOMES AND RESULTS There was a greater increase of FMD, IL-10 and reduction of TNF-alpha/ IL-10, 2 h and 5 days after the G-CSF treatment compared to placebo. Although, CRP and TNF-alpha were higher, TNF-alpha/IL-10 was lower at the end of G-CSF treatment compared to placebo. Improvement of FMD was related to changes of IL-10 and TNF-alpha/IL-10. CONCLUSIONS Treatment with G-CSF improves endothelial function in vivo, possibly by shifting the balance between the pro- and anti-inflammatory cytokines.

中文翻译：

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用粒细胞集落刺激因子治疗与内皮功能的改善有关。

原发性粒细胞集落刺激因子（G-CSF）用于动员骨髓和内皮祖细胞，尽管G-CSF诱导的炎症可能引起内皮功能障碍。我们检查了G-CSF对内皮，C反应蛋白（CRP），肿瘤坏死因子-α（TNF-α）和抗炎细胞因子即白介素10（IL-10）的影响。研究设计我们研究了60例乳腺癌患者，这些患者被随机分配到皮下G-CSF（5微克/千克），辅助化疗（n = 40）或安慰剂（n = 20）后od连续5天。实验干预我们通过超声检查，CRP，TNF-α，IL-10以及血浆TNF-α/ IL-10比值在之前，之后2小时和5天后测量肱动脉的血流介导扩张（FMD％） G-CSF或安慰剂治疗。主要结果和结果与安慰剂相比，G-CSF治疗后2小时和5天，FMD，IL-10和TNF-alpha / IL-10的降低更大。尽管CRP和TNF-α较高，但与安慰剂相比，G-CSF治疗结束时TNF-α/ IL-10较低。FMD的改善与IL-10和TNF-α/ IL-10的变化有关。结论用G-CSF治疗可改善体内内皮功能，可能是通过改变促炎和抗炎细胞因子之间的平衡来实现的。