Metamorphosis, a critical stage in the development of toads and frogs, involves rapid levels of morphological change. In the current study, we have used microarray analysis to identify shifts in gene expression between tadpole and toadlet stages of the cane toad, Bufo (Chaunus) marinus. Here, we report on nine genes that show the greatest induction during metamorphosis; the gut-associated gastrokine and trefoil factor, blood components haemoglobins alpha/beta, apolipoprotein and serum albumin, a nasal gene olfactomedin, a lens gene gamma-crystallin, and a novel gene with low homology to frog harderin. We present both temporal and spatial expression patterns of these genes identified in developing and adult cane toads. This study extends our knowledge of the molecular basis of toad metamorphosis, and not only offers insights to the genes induced during the general remodelling that occurs but also reveals possible targets for control and manipulation of amphibian pest species, for example, the cane toad in Australia.

中文翻译：

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在 Cane Toad, Bufo (Chaunus) marinus 的早期发育阶段诱导的基因。

变形是蟾蜍和青蛙发育的关键阶段，涉及快速的形态变化。在当前的研究中，我们使用微阵列分析来确定甘蔗蟾蜍、蟾蜍 (Chaunus) marinus 的蝌蚪和蟾蜍阶段之间基因表达的变化。在这里，我们报告了在变态过程中表现出最大诱导作用的九个基因；肠道相关的胃因子和三叶因子、血液成分血红蛋白 α/β、载脂蛋白和血清白蛋白、鼻基因 olfactomedin、晶状体基因 γ-晶状体蛋白和与青蛙 hardin 具有低同源性的新基因。我们展示了在发育中和成年甘蔗蟾蜍中鉴定的这些基因的时间和空间表达模式。这项研究扩展了我们对蟾蜍变态分子基础的认识，