BACKGROUND Multiple myeloma (MM) is a clonal B-cell disorder with many immunological disturbances. The aim of this work was to assess whether some of food antigens contribute to the imbalance of immune response by screening the sera of MM patients for their immunoreactivity to food constituent gliadin, to tissue transglutaminase-2 (tTG-2) and to Ro/SSA antigen.Sera from 61 patients with MM in various stages of disease, before, or after some cycles of conventional therapy were analyzed by commercial Binding Site ELISA tests. The control group consisted of 50 healthy volunteers. Statistical analysis of data obtained was performed by Mann Whitney Test. RESULTS The higher serum IgA immunoreactivity to gliadin was found in 14/56 patients and in one of control people. The enhanced serum IgG immunoreactivity to gliadin was found in only two of tested patients and in two controls. The enhanced IgA immunoreactivity to tTG-2 was found in 10/49 patients' sera, while 4/45 patients had higher serum IgG immunoreactivity. The enhanced serum IgG immunoreactivity to RoSSA antigen was found in 9/47 analyzed MM patients' sera. Statistical analysis of data obtained revealed that only the levels of anti-tTG-2 IgA immunoreactivity in patients with MM were significantly higher than these obtained in healthy controls (P < 0.02) CONCLUSION Data obtained showed the existence of the enhanced serum immunoreactivity to gliadin, tTG-2 and Ro/SSA antigens in some patients with MM. These at least partially could contribute to the immunological imbalance frequently found in this disease.

中文翻译：

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一些多发性骨髓瘤患者存在对麦醇溶蛋白和组织转谷氨酰胺酶的体液免疫反应性。


背景技术多发性骨髓瘤（MM）是一种具有多种免疫紊乱的克隆性B细胞疾病。这项工作的目的是通过筛查 MM 患者血清对食物成分麦醇溶蛋白、组织转谷氨酰胺酶 2 (tTG-2) 和 Ro/SSA 的免疫反应性，评估某些食物抗原是否会导致免疫反应失衡。通过商业结合位点 ELISA 测试对 61 名处于不同疾病阶段、常规治疗周期之前或之后的 MM 患者的血清进行了分析。对照组由 50 名健康志愿者组成。通过曼惠特尼检验对获得的数据进行统计分析。结果 14/56 名患者和 1 名对照人群中发现对麦醇溶蛋白的血清 IgA 免疫反应性较高。仅在两名受试患者和两名对照组中发现对麦醇溶蛋白的血清 IgG 免疫反应性增强。 10/49名患者的血清中发现对tTG-2的IgA免疫反应性增强，而4/45名患者的血清IgG免疫反应性较高。在 9/47 分析的 MM 患者血清中发现对 RoSSA 抗原的血清 IgG 免疫反应性增强。对获得的数据进行统计分析表明，只有 MM 患者的抗 tTG-2 IgA 免疫反应水平显着高于健康对照者 (P < 0.02) 结论 获得的数据表明存在对麦醇溶蛋白的血清免疫反应性增强、 tTG-2 和 Ro/SSA 抗原存在于一些 MM 患者中。这些至少部分可能导致这种疾病中常见的免疫失衡。