A histological study on the tissue of nonunion of tibias of two young patients was performed to evaluate the ability of cells to start the mineralization of the matrix. The observations can be summarized as follows: 1) Tissue vessels often appear occluded by thrombotic material; 2) Fibroblasts and chondrocytes found in the nonunion tissue seemed normal, with a good secretion apparatus; 3) The cell membranes were able to produce matrix vesicles; 4) Matrix vesicles and cell membrane looked positive to ALPase reaction, 5) Hydroxyapatite crystals could be observed in the cell matrix or inside matrix vesicles. It may be concluded that cells populating nonunion tissue are well equipped to induct the mineralization of the matrix, but the absence of a blood supply, enough to bring them a normal calcium amount, is the real reason for the nonunion.

中文翻译：

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人类骨不连的超微结构方面。

对两名年轻患者的胫骨骨不连组织进行了组织学研究，以评估细胞开始基质矿化的能力。观察结果可归纳如下：1）组织血管经常被血栓性物质阻塞；2）骨不连组织中发现的成纤维细胞和软骨细胞正常，分泌物良好。3）细胞膜能够产生基质囊泡；4）基质囊泡和细胞膜对ALPase反应呈阳性，5）在细胞基质或基质囊泡内部可观察到羟基磷灰石晶体。可以得出结论，填充骨不连组织的细胞具备良好的条件来诱导基质矿化，但是缺乏血液供应足以使它们达到正常的钙含量，才是造成骨不连的真正原因。