A description is provided for the effects of various concentrations of NaCl, MgC12, and urea on the precipitation of native and denatured DNA by histones. The solubility of complexes between total histones and fractions F1, F2a, F2b, and F3 with denatured and partially denatured DNA was greater than that of the complexes between histones and native DNA. The complexes formed between histones and denatured DNA were soluble in excess histones, unlike those formed between histones and native DNA. Electrophoresis of the individual histone fractions through a polyacrylamide gel layer containing DNA led to the determination of the amount of histones bound to native and denatured DNA under conditions of saturation (0.04 ionic strength). It was established that 1 mug of native DNA binds 2.4, 2.8, and 2.5 mug of histones F1, F2a, F2b and F3, respectively. The denatured DNA binds 1.4-1.5 times less of each histone fraction than does native DNA, but the binding seems stronger. It has been demonstrated that the histones inhibit to a lesser extent the template activity of denatured and partially denatured (about 5% disruption of hydrogen bonds) DNA in comparison with native DNA in an RNA polymerase system. It has been suggested that the properties of the complexes formed between histones and denatured or partially denatured DNA, may underlie the control mechanism for genome activity in the cells of higher organisms.

中文翻译：

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组蛋白与天然和变性DNA的相互作用。

提供了有关各种浓度的NaCl，MgCl2和尿素对组蛋白沉淀天然和变性DNA的影响的描述。总组蛋白与级分F1，F2a，F2b和F3之间具有变性和部分变性DNA的复合物的溶解度大于组蛋白和天然DNA之间的复合物的溶解度。组蛋白和变性DNA之间形成的复合物可溶于过量的组蛋白中，这与组蛋白和天然DNA之间形成的复合物不同。通过含有DNA的聚丙烯酰胺凝胶层对各个组蛋白组分进行电泳，从而确定了在饱和条件下（离子强度为0.04），与天然和变性DNA结合的组蛋白的数量。已经确定1杯天然DNA分别结合2.4、2.8和2.5杯组蛋白F1，F2a，F2b和F3。与天然DNA相比，变性的DNA与每个组蛋白的结合少1.4-1.5倍，但结合似乎更牢固。已经证明，与RNA聚合酶系统中的天然DNA相比，组蛋白在较小程度上抑制了变性和部分变性（约5％的氢键破坏）DNA的模板活性。已经提出，在组蛋白和变性或部分变性的DNA之间形成的复合物的性质，可能是高等生物细胞中基因组活性的控制机制的基础。已经证明，与RNA聚合酶系统中的天然DNA相比，组蛋白在较小程度上抑制了变性和部分变性（约5％的氢键破坏）DNA的模板活性。已经提出，在组蛋白与变性或部分变性的DNA之间形成的复合物的性质，可能是高等生物细胞中基因组活性控制机制的基础。已经证明，与RNA聚合酶系统中的天然DNA相比，组蛋白在较小程度上抑制了变性和部分变性（约5％的氢键破坏）DNA的模板活性。已经提出，在组蛋白和变性或部分变性的DNA之间形成的复合物的性质，可能是高等生物细胞中基因组活性的控制机制的基础。