The antiviral activity of total flavonoid extracts from Selaginella moellendorffii Hieron and its main constituents amentoflavone were investigated against coxsackie virus B3 (CVB3). When added during or after viral infection, the extracts and amentoflavone prevented the cytopathic effect (CPE) of CVB3, as demonstrated in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay, with a 50% inhibitory concentration (IC50) from to  μg/mL and to  μg/mL, respectively. KM mice were used as animal models to test the extracts' activity in vivo. Oral administration of the total flavonoid extracts at 300 mg/kg/day significantly reduced mean viral titers in the heart and kidneys as well as mortality after infection for 15 days. The experimental results demonstrate that in vitro and in vivo the model mice infected with CVB3 can be effectively treated by the total flavonoid extracts from Selaginella moellendorffii Hieron.

中文翻译：

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Selaginella moellendorffii Hieron 总黄酮提取物对柯萨奇病毒 B3 的体外和体内抗病毒活性。

研究了从卷柏中提取的总黄酮类化合物及其主要成分丁黄酮对柯萨奇病毒 B3 (CVB3)的抗病毒活性。在病毒感染期间或之后添加时，提取物和 amentoflavone 可防止 CVB3 的细胞病变效应 (CPE)，如 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT) 所证明的比色测定法，从50％抑制浓度（IC50） ，以μ克/毫升和对μ克/毫升，分别。使用 KM 小鼠作为动物模型来测试提取物的体内活性  . 以 300 毫克/千克/天的剂量口服总黄酮提取物可显着降低心脏和肾脏中的平均病毒滴度以及感染 15 天后的死亡率。实验结果表明，莫氏卷柏总黄酮提取物可有效治疗CVB3感染模型小鼠的体外和体内。