Retinoblastoma (RB) is a childhood malignancy caused by inactivation of the RB gene, with neuron-specific enolase (NSE) levels considered as its diagnostic marker. MicroRNAs (miRNAs) have been proven to play a significant role in multiple physiological and pathological processes and several miRNAs were identified as tumor biomarkers in recent studies. In the present study, 65 plasma samples were collected from RB patients and 65 samples from healthy individuals to serve as controls. The miRNA levels were measured via quantitative reverse transcription-polymerase chain reaction and their association with RB was assessed by statistical data analysis and receiver operating characteristic curves. Plasma miRNA (miR)-320, miR-let-7e and miR-21 levels were downregulated in the patient samples, the areas under the curves (AUCs) were 0.548-0.660, whereas the AUCs of combined classifiers were ≥0.990. The plasma miRNA levels, particularly of miR-320, were found to be of value in RB diagnosis and may be considered as novel diagnostic biomarkers.

中文翻译：

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血浆 microRNA-320、microRNA-let-7e 和 microRNA-21 作为检测视网膜母细胞瘤的新型潜在生物标志物。

视网膜母细胞瘤 (RB) 是由 RB 基因失活引起的儿童恶性肿瘤，神经元特异性烯醇化酶 (NSE) 水平被视为其诊断标志物。MicroRNAs (miRNAs) 已被证明在多种生理和病理过程中发挥着重要作用，并且在最近的研究中，一些 miRNAs 被确定为肿瘤生物标志物。在本研究中，从 RB 患者收集 65 份血浆样本，从健康人收集 65 份样本作为对照。通过定量逆转录聚合酶链反应测量 miRNA 水平，并通过统计数据分析和接收器操作特征曲线评估它们与 RB 的关联。血浆 miRNA (miR)-320、miR-let-7e 和 miR-21 水平在患者样本中下调，曲线下面积 (AUC) 为 0.548-0.660，而组合分类器的 AUC ≥ 0.990。血浆 miRNA 水平，特别是 miR-320，被发现在 RB 诊断中具有价值，可被视为新的诊断生物标志物。