Cellular separations are required in many contexts in biochemical and biomedical applications for the identification, isolation, and analysis of phenotypes or samples of interest. Microfluidics is uniquely suited for handling biological samples, and emerging technologies have become increasingly accessible tools for researchers and clinicians. Here, we review advances in the last few years in techniques for microfluidic cell separation and manipulation. Applications such as high-throughput cell and organism phenotypic screening, purification of heterogeneous stem cell populations, separation of blood components, and isolation of rare cells in patients highlight some of the areas in which these technologies show great potential. Continued advances in separation mechanisms and understanding of cellular systems will yield further improvements in the throughput, resolution, and robustness of techniques.

中文翻译：

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微流体细胞分离和操作的进展。


在生物化学和生物医学应用的许多情况下都需要细胞分离来识别、分离和分析感兴趣的表型或样品。微流控技术特别适合处理生物样本，新兴技术已成为研究人员和临床医生越来越容易使用的工具。在这里，我们回顾了过去几年微流体细胞分离和操作技术的进展。高通量细胞和生物体表型筛选、异质干细胞群纯化、血液成分分离以及患者稀有细胞分离等应用突出了这些技术显示出巨大潜力的一些领域。分离机制和对细胞系统理解的不断进步将进一步提高技术的通量、分辨率和稳健性。