Dynamic monitoring of stimulus-evoked inner neural response is important for functional validation of stimulation protocols of retinal prosthetic devices. In this paper, we demonstrate label-free intrinsic optical signal (IOS) imaging of electrically stimulated inner neural response in freshly isolated mouse retinas. While single-pulse stimulation evoked rapid IOS within 20 ms, pulse-train stimulation indicated that the fast IOS response can follow frequency stimulation up to at least 8 Hz. Fast IOS imaging promises a noninvasive method for high resolution examination of electrically evoked retinal response, without artifact contamination of electrical stimulus.

中文翻译：

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电刺激视网膜内神经反应的动态内在光信号监测

刺激诱发的内部神经反应的动态监测对于视网膜假体装置刺激方案的功能验证很重要。在本文中，我们展示了新鲜分离的小鼠视网膜中电刺激内部神经反应的无标记内在光信号 (IOS) 成像。虽然单脉冲刺激在 20 毫秒内引起快速 IOS，但脉冲串刺激表明快速 IOS 响应可以跟随频率刺激高达至少 8 Hz。快速 IOS 成像有望提供一种非侵入性方法，用于对电诱发的视网膜反应进行高分辨率检查，而不会造成电刺激的伪影污染。