We demonstrate intrinsic optical signal (IOS) imaging of intact rat islet, which consists of many endocrine cells working together. A near-infrared digital microscope was employed for optical monitoring of islet activities evoked by glucose stimulation. Dynamic NIR images revealed transient IOS responses in the islet activated by low-dose (2.75 mM) and high-dose (5.5 mM) glucose stimuli. Comparative experiments and quantitative analysis indicated that both glucose metabolism and calcium/insulin dynamics might contribute to the observed IOS responses. Further investigation of the IOS imaging technology may provide a high resolution method for ex vivo functional examination of the islet, which is important for advanced study of diabetes associated islet dysfunctions and for improved quality control of donor islets for transplantation.

中文翻译：

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使用瞬态固有光信号对葡萄糖诱发的大鼠胰岛活动进行功能成像

我们展示了完整大鼠胰岛的内在光学信号 (IOS) 成像，该胰岛由许多协同工作的内分泌细胞组成。采用近红外数字显微镜对葡萄糖刺激引起的胰岛活动进行光学监测。动态 NIR 图像显示由低剂量 (2.75 mM) 和高剂量 (5.5 mM) 葡萄糖刺激激活的胰岛中的瞬时 IOS 响应。比较实验和定量分析表明，葡萄糖代谢和钙/胰岛素动力学可能有助于观察到的 IOS 反应。IOS 成像技术的进一步研究可能为胰岛的离体功能检查提供高分辨率方法，这对于糖尿病相关胰岛功能障碍的高级研究和改善移植供体胰岛的质量控制非常重要。