Intravital microscopy has emerged in the recent decade as an indispensible imaging modality for the study of the microdynamics of biological processes in live animals. Technical advancements in imaging techniques and hardware components, combined with the development of novel targeted probes and new mice models, have enabled us to address long-standing questions in several biology areas such as oncology, cell biology, immunology, and neuroscience. As the instrument resolution has increased, physiological motion activities have become a major obstacle that prevents imaging live animals at resolutions analogue to the ones obtained in vitro. Motion compensation techniques aim at reducing this gap and can effectively increase the in vivo resolution. This paper provides a technical review of some of the latest developments in motion compensation methods, providing organ specific solutions.

中文翻译：

---

用于活体光学显微镜的先进运动补偿方法


近十年来，活体显微镜已成为研究活体动物生物过程微动力学不可或缺的成像方式。成像技术和硬件组件的技术进步，加上新型靶向探针和新小鼠模型的开发，使我们能够解决肿瘤学、细胞生物学、免疫学和神经科学等多个生物学领域长期存在的问题。随着仪器分辨率的提高，生理运动活动已成为阻碍以类似于体外获得的分辨率对活体动物进行成像的主要障碍。运动补偿技术旨在减少这种差距，并能有效提高体内分辨率。本文对运动补偿方法的一些最新发展进行了技术回顾，提供了针对器官的解决方案。