A novel route was introduced to synthesize dense polyacrylamide (PAM) onto the glass slide surface. To investigate the surface chemistry of the PAM on the glass slides, X-ray photoelectron spectroscopy (XPS) was utilized to obtain detailed chemical state information on the PAM layer constituents. The XPS peak data were consistent with the presented model of the PAM on the glass slide surface. Scanning electron microscopy and atomic force microscope data indicated the presence of PAM on the glass slides, which consist of nodules. The results showed that PAM was successfully immobilized onto glass slides with a two-tier structure under aqueous condition and a monolayer structure under anhydrous condition. Compared with those under aqueous condition, the controllability of the molecular layer on glass slides and the reproducibility under anhydrous condition were much better, which makes anhydrous condition an advisable condition for the study of the reaction mechanisms of glass slides modified by PAM.

中文翻译：

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聚丙烯酰胺在载玻片上的可控固定：合成和表征


引入了一种在载玻片表面合成致密聚丙烯酰胺（PAM）的新路线。为了研究载玻片上 PAM 的表面化学，利用 X 射线光电子能谱 (XPS) 来获取 PAM 层成分的详细化学状态信息。 XPS 峰值数据与载玻片表面上的 PAM 模型一致。扫描电子显微镜和原子力显微镜数据表明载玻片上存在 PAM，载玻片由结节组成。结果表明，PAM成功固定在载玻片上，在水相条件下为两层结构，在无水条件下为单层结构。与水相条件下相比，无水条件下载玻片分子层的可控性和重现性要好得多，这使得无水条件成为研究PAM改性载玻片反应机理的理想条件。