Self-interaction of an antibody may lead to aggregation, low solubility or high viscosity. Rapid identification of highly developable leads remains challenging, even though progress has been made with the introduction of techniques such as self-interaction chromatography (SIC) and cross-interaction chromatography (CIC). Here, we report a high throughput method to detect antibody clone self-interaction (CSI) using bio-layer interferometry (BLI) technology. Antibodies with strong self-interaction responses in the CSI-BLI assay also show delayed retention times in SIC and CIC. This method allows hundreds of candidates to be screened in a matter of hours with minimal material consumption.

抗体的自相互作用可能导致聚集、低溶解度或高粘度。尽管自相互作用色谱 (SIC) 和交叉相互作用色谱 (CIC) 等技术的引入已经取得了进展，但快速鉴定高度可开发的先导物仍然具有挑战性。在这里，我们报告了一种使用生物层干涉测量 (BLI) 技术检测抗体克隆自相互作用 (CSI) 的高通量方法。在 CSI-BLI 测定中具有强自相互作用反应的抗体在 SIC 和 CIC 中也显示延迟的保留时间。这种方法允许在几个小时内以最少的材料消耗筛选数百名候选人。