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Transcriptome sequencing as a platform to elucidate molecular components of the diapause response in the Asian tiger mosquitoAedes albopictus
Physiological Entomology ( IF 1.5 ) Pub Date : 2013-05-06 , DOI: 10.1111/phen.12016
Monica F Poelchau 1 , Julie A Reynolds , David L Denlinger , Christine G Elsik , Peter A Armbruster
Affiliation  

Diapause has long been recognized as a crucial ecological adaptation to spatio‐temporal environmental variation. More recently, rapid evolution of the diapause response has been implicated in response to contemporary global warming and during the range expansion of invasive species. Although the molecular regulation of diapause remains largely unresolved, rapidly emerging next‐generation sequencing (NGS) technologies provide exciting opportunities for addressing this longstanding question. In the present study, a new assembly from life‐history stages relevant to diapause in the Asian tiger mosquito Aedes albopictus (Skuse) is reported, along with unique methods for the analysis of NGS data and transcriptome assembly. A digital normalization procedure that significantly reduces the computational resources required for transcriptome assembly is evaluated. Additionally, a method for protein reference‐based and genomic reference‐based merged assembly of 454 and Illumina reads is described. Finally, a gene ontology analysis is presented, which creates a platform for identifying the physiological processes associated with diapause. Taken together, these methods provide valuable tools for analyzing the transcriptional underpinnings of many complex phenotypes, including diapause, and provide a basis for determining the molecular regulation of diapause in Ae. albopictus.

中文翻译:

转录组测序作为阐明亚洲虎蚊白纹伊蚊滞育反应分子成分的平台

滞育长期以来被认为是对时空环境变化的重要生态适应。最近,滞育反应的快速演变与当代全球变暖和入侵物种范围扩大期间的反应有关。尽管滞育的分子调控在很大程度上仍未得到解决,但快速出现的下一代测序 (NGS) 技术为解决这一长期存在的问题提供了令人兴奋的机会。在本研究中,报告了与亚洲虎蚊白纹伊蚊(Skuse)滞育相关的生命史阶段的新组装,以及用于分析 NGS 数据和转录组组装的独特方法。评估了显着减少转录组组装所需计算资源的数字标准化程序。此外,还描述了一种基于蛋白质参考和基于基因组参考的 454 和 Illumina 读数的合并组装方法。最后,介绍了基因本体分析,它创建了一个平台,用于识别与滞育相关的生理过程。总之,这些方法为分析包括滞育在内的许多复杂表型的转录基础提供了有价值的工具,并为确定 Ae 中滞育的分子调控提供了基础。白线虫。它创建了一个平台,用于识别与滞育相关的生理过程。总之,这些方法为分析包括滞育在内的许多复杂表型的转录基础提供了有价值的工具,并为确定 Ae 中滞育的分子调控提供了基础。白线虫。它创建了一个平台,用于识别与滞育相关的生理过程。总之,这些方法为分析包括滞育在内的许多复杂表型的转录基础提供了有价值的工具,并为确定 Ae 中滞育的分子调控提供了基础。白线虫。
更新日期:2013-05-06
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