The objective of the current study was to investigate the protective effects of hesperidin against oxidative stress, altered cytokines levels and histological changes in rats induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were divided randomly into four equal groups (Control, TCDD, hesperidin and TCDD+hesperidin). TCDD and hesperidin were given by gavage, dissolved in corn oil at doses of 2 μ/kg/week and 50 mg/kg/day respectively. The blood and tissue samples were taken from all rats on the 60(th) day, to be analyzed for the determination of oxidative stress, histological changes and cytokine levels. The results indicated that hesperidin prevented oxidative damage caused by TCDD via decrease lipid peroxidation and increased antioxidant defense systems. It also reversed the histological damage induced by TCDD. Although, TCDD led to a significant increase in TNF-α and IL-1β levels, hesperidin treatment was able to normalize these values in rats. In conclusion, it was shown that TCDD caused adverse effects as regards cytokine levels, histological alterations and oxidative stress in rats. However, hesperidin treatment mitigated these toxic effects. These results suggest that hesperidin could play a protective role against TCDD toxicity.

中文翻译：

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在大鼠中口服橙皮苷（柑桔类黄酮）可抵消氧化应激，炎性细胞因子的产生以及因摄入2、3、7、8-四氯二苯并-对-二恶英（TCDD）而引起的肝毒性。

本研究的目的是研究橙皮苷对2,3,7,8-四氯二苯并-p-二恶英（TCDD）诱导的大鼠氧化应激，细胞因子水平改变和组织学变化的保护作用。将大鼠随机分为四个相等的组（对照组，TCDD，橙皮苷和TCDD +橙皮苷）。TCDD和橙皮苷通过管饲法给药，分别以2μ/ kg /周和50 mg / kg /天的剂量溶于玉米油中。在第60天从所有大鼠中采集血液和组织样本，进行分析以确定氧化应激，组织学变化和细胞因子水平。结果表明橙皮苷通过减少脂质过氧化作用和增加抗氧化防御系统来预防TCDD引起的氧化损伤。它还逆转了由TCDD引起的组织学损伤。虽然，TCDD导致TNF-α和IL-1β水平显着增加，橙皮苷治疗能够使大鼠中的这些值正常化。总之，已经表明TCDD在细胞因子水平，组织学改变和氧化应激方面引起了不良反应。然而，橙皮苷治疗减轻了这些毒性作用。这些结果表明橙皮苷可能对TCDD毒性起保护作用。