Direct and fast (10s of seconds) deposition of flame-made, high surface-area aerosol films on polymers and polymeric microfluidic devices is demonstrated. Uniform TiO2 nanoparticle films were deposited on cooled Poly(methyl methacrylate) (PMMA) substrates by combustion of titanium(IV) isopropoxide (TTIP) - xylene solution sprays. Films were mechanically stabilized by in-situ annealing with a xylene spray flame. Plasma-etched microfluidic chromatography columns, comprising parallel microchannels were also coated with such nanoparticle films without any microchannel deformation. These microcolumns were successfully used in metal-oxide affinity chromatography (MOAC) to selectively trap phosphopeptides on these high surface-area nanostructured films. The chips had a high capacity retaining 1.2 μg of standard phosphopeptide. A new extremely fast method is developed for MOAC microchip stationary phase fabrication with applications in proteomics.

中文翻译：

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聚合物和聚合物微流控装置上的二氧化钛纳米颗粒薄膜的火焰气溶胶沉积，用于片上磷酸肽富集

演示了在聚合物和聚合物微流体装置上直接快速（10 秒）沉积火焰制成的高表面积气溶胶薄膜。通过燃烧异丙醇钛 (IV) (TTIP) - 二甲苯溶液喷雾，将均匀的 TiO2 纳米颗粒薄膜沉积在冷却的聚甲基丙烯酸甲酯 (PMMA) 基底上。通过用二甲苯喷射火焰进行原位退火，使薄膜机械稳定。包括平行微通道的等离子蚀刻微流体色谱柱也涂有这样的纳米颗粒膜，而没有任何微通道变形。这些微柱已成功用于金属氧化物亲和色谱 (MOAC)，以选择性地捕获这些高表面积纳米结构薄膜上的磷酸肽。该芯片具有高容量，可保留 1.2 μg 标准磷酸肽。