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In vitro evaluation of the capacity of zeolite and bentonite to adsorb aflatoxin B1 in simulated gastrointestinal fluids.
Mycotoxin Research ( IF 2.6 ) Pub Date : 2008-09-01 , DOI: 10.1007/bf03032338
N Q Thieu 1 , H Pettersson
Affiliation  

Anin vitro study using single concentration and isotherm adsorption was carried out to evaluate the capacity of Vietnamese produced zeolite and bentonite to adsorb aflatoxin B1 (AFB1) in simulated gastrointestinal fluids (SGFs), and a commercial sorbent hydrated sodium calcium aluminosilicate (HSCAS) was used as reference. In this study, AFB1 solution was mixed with sorbents (0.3, 0.4 and 0.5% w/v) in SGFs at pH 3 and pH 7 and shaken for 8 h, centrifuged and the supernatant measured by Vicam fluorometer. Adsorption of AFB1 onto zeolite and bentonite varied according to the pH of SGFs and was lower than HSCAS. Linearity between the increased amount of AFB1 adsorbed on sorbents and the decrease of sorbent concentration was observed for bentonite and HSCAS, except for zeolite in SGFs at pH 7. The observed maximum amounts of AFB1 adsorbed on bentonite and HSCAS were 1.54 and 1.56 mg/g, respectively. The adsorption capacities of bentonite and HSCAS for AFB1 were 12.7 and 13.1 mg/g, respectively, from fitting the data to the Freundlich isotherm equation. Improvement in processing and purification for bentonite is needed to enhance the surface area, which would probably result in better adsorptive capacity for this sorbent.

中文翻译:

沸石和膨润土在模拟胃肠液中吸附黄曲霉毒素 B1 的能力的体外评价。

使用单一浓度和等温线吸附进行体外研究,以评估越南生产的沸石和膨润土对模拟胃肠液 (SGF) 中黄曲霉毒素 B1 (AFB1) 的吸附能力,并使用商业吸附剂水合铝硅酸钙钠 (HSCAS)作为参考。在这项研究中,AFB1 溶液与吸附剂(0.3、0.4 和 0.5% w/v)在 SGFs 中混合,pH 为 3 和 pH 7,摇晃 8 小时,离心,并通过 Vicam 荧光计测量上清液。AFB1 在沸石和膨润土上的吸附根据 SGFs 的 pH 值而变化,并且低于 HSCAS。对于膨润土和 HSCAS 观察到吸附在吸附剂上的 AFB1 量增加与吸附剂浓度降低之间存在线性关系,但 SGF 中的沸石在 pH 值为 7 时除外。观察到的最大 AFB1 吸附在膨润土和 HSCAS 上的量分别为 1.54 和 1.56 mg/g。根据将数据拟合到 Freundlich 等温方程,膨润土和 HSCAS 对 AFB1 的吸附容量分别为 12.7 和 13.1 mg/g。需要改进膨润土的加工和纯化以提高表面积,这可能会导致这种吸附剂的吸附能力更好。
更新日期:2019-11-01
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