A HPLC method for the determination of ergometrine, ergotamine, ergocristine, α-ergocryptine and ergocornine in cereals for animal feed and in mixed feed with high cereal content was developed. Samples were extracted under acidic conditions using a mixture of phosphoric acid and acetonitrile, the extract purified with solid phase extraction cartridges (strong cation exchange), and ergot alkaloids detected after gradient elution on a C18 column by HPLC with fluorescence detection. Detection and determination limits for each individual alkaloid were at 5 (μ/kg and 10 (μg/kg, respectively. With this method, high recovery (82-120%) and good reproducibility was achieved for wheat, rye and mixed feeds, at a sum of total determined alkaloids of < 500 (μg/kg. This method was used to analyse Bavarian feeds (n=124) over three years (2005-2007), and ergot alkaloids were detected in 91 % of the samples. The majority of positive samples had ergot alkaloid contents of < 250 μg/kg, the median alkaloid level was at 70 (μg/kg. The maximum sum of total determined alkaloids exceeded 1000 (μg/kg in wheat, triticale, rye, and mixed feeds, the highest result was obtained for mixed feed (4880 (μg/kg).

中文翻译：

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高效液相色谱法测定饲料中麦角生物碱。

建立了测定动物饲料和高谷物混合饲料中麦角新碱、麦角胺、麦角新碱、α-麦角隐亭和麦角角碱的 HPLC 方法。样品在酸性条件下使用磷酸和乙腈的混合物进行提取，提取物用固相萃取柱（强阳离子交换）纯化，并在 C18 柱上梯度洗脱后通过荧光检测的 HPLC 检测麦角生物碱。每种生物碱的检测限和测定限分别为 5 (μ/kg 和 10 (μg/kg)。使用这种方法，小麦、黑麦和混合饲料的回收率高 (82-120%) 和良好的重现性，在测定的总生物碱总量 < 500 (μg/kg。该方法用于分析巴伐利亚饲料 (n=124) 三年 (2005-2007)，91% 的样品中检出麦角生物碱。大多数阳性样品麦角生物碱含量< 250 μg/kg，生物碱中位数为70（μg/kg。总测定生物碱的最大总量超过1000（小麦、黑麦、黑麦和混合小麦中的μg/kg）饲料中，混合饲料获得的结果最高（4880 (μg/kg)）。