Stenotrophomonas maltophilia strain C6, capable of utilizing phenanthrene as a sole source of carbon and energy, was isolated from creosote-contaminated sites at Hilo, Hawaii. Twenty-two metabolites of phenanthrene, covering from dihydrodiol to protocatechuic acid, were isolated and characterized. Phenanthrene was degraded via an initial dioxygenation on 1,2-, 3,4-, and 9,10-C, where the 3,4-dioxygenation and subsequent metabolisms were most dominant. The metabolic pathways were further branched by ortho- and meta-cleavage of phenanthrenediols to produce 1-hydroxy-2-naphthoic acid, 2-hydroxy-1-naphthoic acid, and naphthalene-1,2-dicarboxylic acid. These intermediates were then transformed to naphthalene-1,2-diol. 1-Hydroxy-2-naphthoic acid was also degraded via a direct ring cleavage. Naphthalene-1,2-diol underwent primarily ortho-cleavage to produce trans-2-carboxycinnamic acid and then to form phthalic acid, 4,5-dihydroxyphthalic acid and protocatechuic acid. Accumulation of salicylic acid in prolonged incubation indicated that a limited extent of meta-cleavage of naphthalene-1, 2-diol also occurred. This is the first study of detailed phenanthrene metabolic pathways by Stenotrophomonas maltophilia.

中文翻译：

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嗜麦芽窄食单胞菌C6对菲的多种降解途径

从夏威夷希洛的杂酚油污染地点分离出嗜麦芽窄食单胞菌 C6 菌株，该菌株能够利用菲作为唯一的碳和能量来源。从二氢二醇到原儿茶酸，菲的 22 种代谢物被分离和表征。菲通过 1,2-、3,4- 和 9,10-C 上的初始双氧合降解，其中 3,4-双氧合和随后的代谢占主导地位。代谢途径通过菲二醇的邻位和间位裂解进一步分支，以产生 1-羟基-2-萘甲酸、2-羟基-1-萘甲酸和萘-1,2-二羧酸。然后将这些中间体转化为萘-1,2-二醇。1-Hydroxy-2-naphthoic 酸也可通过直接开环降解。萘-1，2-二醇主要经过邻位裂解产生反式-2-羧基肉桂酸，然后形成邻苯二甲酸、4,5-二羟基邻苯二甲酸和原儿茶酸。长时间孵育中水杨酸的积累表明，naphthalene-1, 2-diol 也发生了有限程度的间位裂解。这是嗜麦芽窄食单胞菌对详细菲代谢途径的首次研究。