Recently, the use of recombinant full-length amelogenin protein in combination with fluoride has shown promising results in the formation of densely packed enamel-like structures. In this study, amelogenin (rP172)-releasing hydrogels containing calcium, phosphate, and fluoride were investigated for remineralization efficacy using in vitro early enamel caries models. The hydrogels were applied to artificial caries lesions on extracted human third molars, and the remineralization efficacy was tested in different models: static gel remineralization in the presence of artificial saliva, pH cyclic treatment at pH 5.4 acetic buffer and pH 7.3 gel remineralization, and treatment with multispecies oral biofilms grown in a continuous flowing constant-depth film fermenter. The surface microhardness of remineralized enamel increased significantly when amelogenin was released from hydrogel. No cytotoxicity was observed when periodontal ligament cells were cultured with the mineralized hydrogels.

中文翻译：

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新型釉原蛋白释放水凝胶用于牙釉质人工龋齿的再矿化


最近，重组全长釉原蛋白与氟化物的结合使用在形成致密的釉质样结构方面显示出有希望的结果。在这项研究中，使用体外早期牙釉质龋齿模型研究了含有钙、磷酸盐和氟化物的牙釉蛋白（rP172）释放水凝胶的再矿化功效。将水凝胶应用于拔除的人第三磨牙上的人工龋病灶，并在不同模型中测试再矿化功效：人工唾液存在下的静态凝胶再矿化、pH 5.4乙酸缓冲液和pH 7.3凝胶再矿化的pH循环处理以及治疗在连续流动的恒定深度薄膜发酵罐中生长多种物种的口腔生物膜。当釉原蛋白从水凝胶中释放时，再矿化牙釉质的表面显微硬度显着增加。当用矿化水凝胶培养牙周膜细胞时，没有观察到细胞毒性。