Alismatis Rhizoma is a perennial herb originating from the rhizomes of Alisma orientalis (Sam) Juzep and the same species which have been used to treat seborrheic dermatitis, eczema, polydipsia, and pedal edema. We aimed to determine the concentrations of the compounds alisol B and alisol B acetate present in a sample of the herb using high-performance liquid chromatography coupled with a photodiode array detector. We selected methanol as the optimal solvent considering the structures of alisol B and alisol B acetate. We estimated the proportion of alisol B and alisol B acetate in a standard extract to be 0.0434% and 0.2365% in methanol, respectively. To optimize extraction, we employed response surface methodology to determine the yields of alisol B and alisol B acetate, which mapped out a central composite design consisting of 15 experimental points. The extraction parameters were time, concentration, and sample weight. The predicted concentration of alisol B derivatives was estimated to be 0.2388% under the following conditions: 81 min of extraction time, 76% of methanol concentration, and 1.52 g of sample weight.

中文翻译：

---

使用响应表面方法优化泽泻根中 ALISOL B 和 ALISOL B 醋酸盐的提取条件

泽泻根茎是一种多年生草本植物，来源于泽泻的根茎，与用于治疗脂溢性皮炎、湿疹、烦渴和足部水肿的同一物种。我们旨在使用高效液相色谱法和光电二极管阵列检测器确定存在于草药样品中的化合物 alisol B 和 alisol B 醋酸盐的浓度。考虑到 alisol B 和 alisol B 醋酸盐的结构，我们选择甲醇作为最佳溶剂。我们估计标准提取物中的 alisol B 和 alisol B 醋酸盐的比例在甲醇中分别为 0.0434% 和 0.2365%。为了优化提取，我们采用响应面方法来确定 alisol B 和 alisol B 醋酸盐的产率，它绘制了一个由 15 个实验点组成的中心复合设计。提取参数是时间、浓度和样品重量。在以下条件下，估计 alisol B 衍生物的预测浓度为 0.2388%：提取时间为 81 分钟，甲醇浓度为 76%，样品重量为 1.52 克。