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Use of bacteria for rapid, pH-neutral, hydrolysis of the model hydrophobic carboxylic acid esterp-nitrophenyl picolinate
Biocatalysis and Biotransformation ( IF 1.4 ) Pub Date : 2012-07-04 , DOI: 10.3109/10242422.2012.702269
Alexandra E Forest 1 , Gordon G Goldstine , Yann Schrodi , Sean R Murray
Affiliation  

Abstract Caulobacter crescentus, Escherichia coli and Bacillus subtilis cultures promote the hydrolysis of the model ester p-nitrophenyl picolinate (PNPP) at neutral pH with high efficiency. Hydrolysis is related to cell concentration, while the interaction of PNPP with both bacterial cells and their extracellular molecules is required for a maximum rate of PNPP hydrolysis in C. crescentus cultures. Furthermore, C. crescentus cultures hydrolyse PNPP at concentrations useful in synthetic chemistry.

中文翻译:

使用细菌快速、pH 中性、水解模型疏水性羧酸酯对硝基苯基吡啶甲酸酯

摘要 Caulobacter crescentus、大肠杆菌和枯草芽孢杆菌在中性pH 条件下高效促进了模型酯对硝基苯基吡啶甲酸酯(PNPP) 的水解。水解与细胞浓度有关,而 PNPP 与细菌细胞及其细胞外分子的相互作用是 C. crescentus 培养物中 PNPP 水解的最大速率所必需的。此外,C. crescentus 培养物以可用于合成化学的浓度水解 PNPP。
更新日期:2012-07-04
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