Many studies have been performed on venomous peptides derived from animals. However, little of this research has focused on peptides from centipede venoms. Here, a venom gland cDNA library was successfully constructed for the centipede Scolopendra subspinipes mutilans. A new cDNA encoding the precursor of a venom peptide, named SsmTx, was cloned from the venomous gland cDNA library of the centipede S. subspinipes mutilans. The full-length SsmTx cDNA sequence is 465 nt, including a 249 nt ORF, a 45 nt 5' UTR and a 171 nt 3' UTR. There is a signal tail AATAAA 31 nt upstream of the poly (A) tail. The precursor nucleotide sequence of SsmTx encodes a signal peptide of 25 residues and a mature peptide of 57 residues, which is bridged by two pairs of disulfide bonds. SsmTx displays a unique cysteine motif that is completely different from that of other venomous animal toxins. This is the first reported cDNA sequence encoding a venom peptide from the centipede S. subspinipes mutilans.

中文翻译：

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分子克隆和特征化的新S编码蛇毒S的毒肽的肽序列。

已经对源自动物的有毒肽进行了许多研究。但是，这项研究很少集中在cent毒液中的肽上。在这里，成功地为forScolopendra subspinipes mutilans构建了毒腺cDNA文库。从the的S. subspinipes mutilans的有毒腺cDNA库中克隆了一种新的编码毒液肽前体的cDNA，称为SsmTx。全长SsmTx cDNA序列为465nt，包括249nt的ORF，45nt的5'UTR和171nt的3'UTR。聚（A）尾部的上游有一个信号尾部AATAAA 31 nt。SsmTx的前体核苷酸序列编码25个残基的信号肽和57个残基的成熟肽，该肽被两对二硫键桥接。SsmTx显示出独特的半胱氨酸基序，与其他有毒动物毒素完全不同。这是第一个报道的编码encoding m亚种S.spinipes mutilans毒液肽的cDNA序列。