A review with over 100 references describes the recent applications of ion-molecule reactions to the study of gas-phase protonated peptides and proteins. The topic is focused specifically on the proton transfer and hydrogen-deuterium exchange reactions of amino acids, peptides, and proteins. A brief background is given of the various methods used for assigning proton affinities and gas-phase basicities. The methods used for measuring the kinetics of deuterium incorporation of charged ion in the presence of a background pressure of deuterating reagents are also described. Ion-molecule reactions are used to determine, among other things, the gas-phase basicities and proton affinities of amino acids, peptides, and proteins, the sites of protonation, intra- and intermolecular interactions, and conformational differences and changes in gas-phase ionic species. Singly charged and multiply charged ions are both covered.

中文翻译：

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离子分子反应作为肽和蛋白质气相结构的探针。

包含100多个参考文献的综述描述了离子分子反应在气相质子化肽和蛋白质研究中的最新应用。该主题专门针对氨基酸，肽和蛋白质的质子转移和氢-氘交换反应。简要介绍了用于分配质子亲和力和气相碱度的各种方法。还描述了用于在氘化试剂的背景压力存在下测量掺入带电离子的氘的动力学的方法。离子分子反应可用于确定氨基酸，多肽和蛋白质的气相碱性和质子亲和力，质子化位点，分子内和分子间相互作用，气相离子物种的构象差异和变化。单电荷和多电荷离子均被覆盖。