Objectives: To determine any association between serum paraoxonase-1 (PON1) activity, protein and coding region genetic polymorphisms and coronary artery calcification (CACS) and to determine factors which modulate serum PON1 in type 2 diabetes (T2DM).Methods and results: 589 patients (419 Caucasian, 120 South Asian, 50 other) from the PREDICT Study were investigated. All patients were asymptomatic for coronary disease and had established T2DM. CACS, lipids, lipoproteins, inflammatory markers, insulin resistance and PON1 activity, concentration and Q192R and L55M genotypes were measured. Independent associations were: 1) PON1 activity negatively with insulin resistance, triglycerides and PON1-55 genotype and positively with PON1-192 genotype; 2) PON1 concentration negatively with Caucasian ethnicity, duration of diabetes and statin use and positively with plasma creatinine and PON1-192 genotype. There was no association between CACS and any of the PON1 activity, concentration or genotype and this finding was not different in the various ethnic groups within the PREDICT study.Conclusion: PON1 is modulated by a number of factors, some of which are reported here for the first time, including ethnicity and insulin resistance in subjects with T2DM. No association between CACS and PON1 was found.

中文翻译：

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Paraoxonase-1 与 2 型糖尿病的冠状动脉钙化无关：来自 PREDICT 研究的结果。

目的：确定血清对氧磷酶-1 (PON1) 活性、蛋白质和编码区遗传多态性与冠状动脉钙化 (CACS) 之间的任何关联，并确定在 2 型糖尿病 (T2DM) 中调节血清 PON1 的因素。方法和结果：对来自 PREDICT 研究的 589 名患者（419 名白种人、120 名南亚人、50 名其他人）进行了调查。所有患者均无冠状动脉疾病症状并已确诊为 T2DM。测量了 CACS、脂质、脂蛋白、炎症标志物、胰岛素抵抗和 PON1 活性、浓度以及 Q192R 和 L55M 基因型。独立关联是：1) PON1 活性与胰岛素抵抗、甘油三酯和 PON1-55 基因型呈负相关，与 PON1-192 基因型呈正相关；2) PON1 浓度与白种人种族、糖尿病持续时间和他汀类药物使用呈负相关，而与血浆肌酐和 PON1-192 基因型呈正相关。CACS 与任何 PON1 活性、浓度或基因型之间都没有关联，而且这一发现在 PREDICT 研究中的不同种族群体中没有差异。结论： PON1 受多种因素调节，其中一些因素首次在此报告，包括 T2DM 受试者的种族和胰岛素抵抗。未发现 CACS 和 PON1 之间的关联。