Mycobacterium aromativorans strain JS19b1(T) can utilize phenanthrene as a sole source of carbon and energy. Strain JS19b1(T) degrades phenanthrene through highly branched metabolic pathways, including dioxygenation on C-1,2, C-3,4 and C-9,10 positions and ring opening by both ortho- and meta-cleavage. The presence of novel metabolic pathways was confirmed by replacement cultivation using synthetic metabolite standards. The metabolites were isolated and identified by gas chromatography-mass spectrometry. Both ortho and meta-cleavage products of 1,2- and 3,4-dihydroxyphenanthrene were detected. Two ortho-cleavage products, 1-[(E)-2-carboxyvinyl]-2-naphthoic acid and 2-[(E)-2-carboxyvinyl]-1-napthoic acid were further metabolized to naphthalene-1,2-dicarboxylic acid and then to 1,2-dihydroxynaphthalene, which can also be produced from the meta-cleavage products hydroxynaphthoic acids. These results suggest that part of the branched pathways is merged into 1,2-dihydroxynaphthalene. The concentrations of the products from C-9,10 dioxygenation were higher than those from other pathways. C-9,10 dioxygenation of phenanthrene produced phthalic acid through decarboxylation and mono-/di-oxygenation. The diverse phenanthrene metabolic pathways in JS19b1(T) give a new insight of the bacterial degradation of polycyclic aromatic hydrocarbons.

中文翻译：

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芳香分枝杆菌 JS19b1T 通过 C-1,2、C-3,4 和 C-9,10 双氧合途径降解菲

芳香分枝杆菌菌株 JS19b1(T) 可以利用菲作为碳和能量的唯一来源。菌株 JS19b1(T) 通过高度分支的代谢途径降解菲，包括 C-1,2、C-3,4 和 C-9,10 位置的双氧合以及通过邻位和间位裂解开环。通过使用合成代谢物标准的替代培养证实了新代谢途径的存在。通过气相色谱-质谱法分离和鉴定代谢物。检测到 1,2-和 3,4-二羟基菲的邻位和间位裂解产物。两种邻位裂解产物 1-[(E)-2-羧基乙烯基]-2-萘甲酸和 2-[(E)-2-羧基乙烯基]-1-萘甲酸进一步代谢为萘-1,2-二甲酸酸，然后到 1,2-二羟基萘，它也可以由间位裂解产物羟基萘甲酸生产。这些结果表明部分支化途径合并为 1,2-二羟基萘。C-9,10 双氧合产物的浓度高于其他途径的产物。菲的 C-9,10 双氧合通过脱羧和单/双氧合产生邻苯二甲酸。JS19b1(T) 中多样化的菲代谢途径为多环芳烃的细菌降解提供了新的见解。10 菲的双氧合通过脱羧和单/双氧合产生邻苯二甲酸。JS19b1(T) 中多样化的菲代谢途径为多环芳烃的细菌降解提供了新的见解。10 菲的双氧合通过脱羧和单/双氧合产生邻苯二甲酸。JS19b1(T) 中多样化的菲代谢途径为多环芳烃的细菌降解提供了新的见解。